The effect of oral antibiotic therapy on bladder functions in an awake rat model of interstitial cystitis
IUGA Academy. CHOI B. Jun 30, 2018; 212972; 348 Topic: Interstitial Cystitis
Ms. BO-HWA CHOI
Ms. BO-HWA CHOI

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348

The effect of oral antibiotic therapy on bladder functions in an awake rat model of interstitial cystitis

Lee, T1;Choi, B2; Yoon, S3

1: Inha University Hospital; 2: INHA UNIVERSITY COLLEGE OF MEDICINE; 3: INHA UNIVERSITY HOSPITAL

Introduction: Interstitial cystitis and bacterial chronic cystitis are difficult to distinguish clinically, and antibiotics are often used empirically in patients with interstitial cystitis. However, the therapeutic effect of antibiotics in patients with interstitial cystitis is still controversial.

Objective: The aim of the study was to investigate the effect of oral antibiotic therapy on the bladder function in an awake rat model of interstitial cystitis.

Methods: A total of 18 female Sprague-Dawley rats were used in this study. In twelve rats, IC was induced by the intravesical instillation of lipopolysaccharide (LPS) following protamine sulfate (PS), as described previously, and the other six rats with intravesical instillation of saline were used as the sham (group A). After this induction, six IC rats was given drinking water that had no antibiotic (group B), and the other six rats were given drinking water containing levofloxacin (group C). After 4 weeks, cystometrograms were obtained in all unanesthetized, unrestrained rats in metabolic cages. The rats were killed just after cystometry. The bladders were removed and weighed.

Results: The IC rats showed no significant difference in pressure parameters including basal pressure (BP), threshold pressure (TP), micturition pressure(MP), volume paratemeters including bladder capacity (BC), micturition volume (MV), residual volume (RV), and micturition interval (MI), compared with the sham group. And there was no significant difference between IC rats with and without antibiotic in all parameters.

Conclusions: Interstitial cystitis is characterized by the symptoms similar to those suffering from bacterial cystitis, such as unexplained chronic pain and irritable voiding symptoms. Therefore, many patients who have been diagnosed with interstitial cystitis have been treated with antibiotics in the course of disease, even if sterile urine is usually found. However, our results showed that antibiotics have no effect on voiding symptoms in patients with true interstitial cystitis, which should be kept in mind when treating those patients.

Reference: Cell Cycle, 2017;16(8):749-758.

Table. General and voiding charateristics of sham and IC rats with or without antibitoics.

Group A

Group B

Group C

Body Wt.gr

215.80±4.55

206.70±2.79

222.50±5.88

Bladder Wt.gr

0.16±0.01

0.17±0.01

0.21±0.02

BP.cmH2O

9.35±1.50

14.23±3.27

14.37±2.23

TP.cmH2O

21.40±1.66

26.34±2.09

41.36±6.92

MP.cmH2O

21.40±1.66

26.34±2.09

41.36±6.92

BC.mL

0.82±0.15

0.70±0.12

0.99±0.10

MV.mL

0.81±0.15

0.70±0.12

0.98±0.10

RV.mL

0.01±0.01

0±0

0.01±0.01

MI.min

4.80±0.65

4.00±0.76

5.50±0.38

Disclosure:

Work supported by industry: no.

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